{"id":9666,"date":"2021-05-06T21:40:53","date_gmt":"2021-05-06T13:40:53","guid":{"rendered":"http:\/\/wp.huhualing.net\/?p=9666"},"modified":"2021-05-06T22:16:50","modified_gmt":"2021-05-06T14:16:50","slug":"%e9%ab%98%e5%8e%8b%e6%b0%a7%e7%96%97%e6%b3%95%e5%8f%af%e5%a2%9e%e5%8a%a0%e7%ab%af%e7%b2%92%e7%9a%84%e9%95%bf%e5%ba%a6%e5%b9%b6%e5%87%8f%e5%b0%91%e7%a6%bb%e4%bd%93%e8%a1%80%e7%bb%86%e8%83%9e%e7%9a%84","status":"publish","type":"post","link":"http:\/\/wp.huhualing.net\/?p=9666","title":{"rendered":"\u9ad8\u538b\u6c27\u7597\u6cd5\u53ef\u589e\u52a0\u7aef\u7c92\u7684\u957f\u5ea6\u5e76\u51cf\u5c11\u79bb\u4f53\u8840\u7ec6\u80de\u7684\u514d\u75ab\u8870\u8001\uff1a\u4e00\u9879\u524d\u77bb\u6027\u8bd5\u9a8c"},"content":{"rendered":"

\u65b0\u95fb<\/strong><\/p>\n

https:\/\/www.medsci.cn\/article\/show_article.do?id=a349203e1892<\/a><\/p>\n

\u7b80\u4ecb<\/strong>\uff1a\u8870\u8001\u7684\u7279\u5f81\u662f\u751f\u7406\u80fd\u529b\u7684\u9010\u6e10\u4e27\u5931\u3002\u5728\u7ec6\u80de\u6c34\u5e73\u4e0a\uff0c\u8870\u8001\u8fc7\u7a0b\u7684\u4e24\u4e2a\u5173\u952e\u6807\u5fd7\u5305\u62ec\u7aef\u7c92\u957f\u5ea6\uff08TL\uff09\u7f29\u77ed\u548c\u7ec6\u80de\u8870\u8001\u3002\u4f7f\u7528\u67d0\u4e9b\u9ad8\u538b\u6c27\u7597\u6cd5\uff08HBOT\uff09\u53cd\u590d\u8fdb\u884c\u7684\u95f4\u6b47\u6027\u9ad8\u6c27\u66b4\u9732\u53ef\u5f15\u8d77\u518d\u751f\u6548\u5e94\uff0c\u8fd9\u79cd\u6548\u5e94\u901a\u5e38\u5728\u7f3a\u6c27\u671f\u95f4\u53d1\u751f\u3002\u5f53\u524d\u7814\u7a76\u7684\u76ee\u7684\u662f\u8bc4\u4f30HBOT\u662f\u5426\u4f1a\u5f71\u54cd\u6b63\u5e38\uff0c\u975e\u75c5\u7406\uff0c\u8001\u9f84\u6210\u5e74\u4eba\u53e3\u4e2d\u7684TL\u548c\u8870\u8001\u7ec6\u80de\u6d53\u5ea6\u3002<\/p>\n

\u65b9\u6cd5\uff1a\u62db\u52df\u4e8635\u540d64\u5c81\u53ca\u4ee5\u4e0a\u7684\u5065\u5eb7\u72ec\u7acb\u751f\u6d3b\u7684\u6210\u5e74\u4eba\uff0c\u6bcf\u5929\u63a5\u53d760\u6b21HBOT\u66b4\u9732\u3002\u5168\u8840\u6837\u54c1\u6536\u96c6\u57fa\u7ebf\uff0c\u572830\u6b21<\/sup>\u548c60\u6b21<\/sup>\uff0c\u6700\u540eHBOT\u4f1a\u8bae\u4e4b\u540e\u4f1a\u8bdd\uff0c1-2\u5468\u3002\u8bc4\u4f30\u5916\u5468\u8840\u5355\u4e2a\u6838\u7ec6\u80de\uff08PBMC\uff09\u7aef\u7c92\u7684\u957f\u5ea6\u548c\u8870\u8001\u3002<\/p>\n

\u7ed3\u679c\uff1aHBOT\u540e\uff0cT\u8f85\u52a9\u7ec6\u80de\uff0cT\u7ec6\u80de\u6bd2\u6027\uff0c\u5929\u7136\u6740\u4f24\u7ec6\u80de\u548cB\u7ec6\u80de\u7684\u7aef\u7c92\u957f\u5ea6\u663e\u7740\u589e\u52a0\u4e8620\uff05\u4ee5\u4e0a\u3002\u6700\u663e\u8457\u53d8\u5316\u662f\u5728B\u7ec6\u80de\uff0c\u5176\u572830\u589e\u52a0\u6ce8\u610f\u5230\u6b21<\/sup>\u4f1a\u8bae\u4e0a\uff0c60\u4e2a<\/sup>\u753125.68\uff05\u00b140.42\uff08p\u503c= 0.007\uff09\uff0c29.39\uff05\u00b123.39\uff08p\u503c= 0.0001\uff09\u548c37.63\uff05\u00b152.73\uff08\u5bf9\u4f1a\u8bdd\u548c\u4ea4\u9ad8\u538b\u6c27= 0.007\uff09\u3002<\/p>\n

HBOT\u540e\u8870\u8001\u7684T\u8f85\u52a9\u7ec6\u80de\u6570\u91cf\u663e\u7740\u51cf\u5c11-37.30\uff05\u00b133.04\uff08P <0.0001\uff09\u3002HBOT\u540eT\u7ec6\u80de\u6bd2\u6027\u8870\u8001\u7ec6\u80de\u767e\u5206\u6bd4\u663e\u7740\u4e0b\u964d-10.96\uff05\u00b112.59\uff08p = 0.0004\uff09\u3002<\/p>\n

\u603b\u4e4b\uff0c\u8be5\u7814\u7a76\u8868\u660e\uff0cHBOT\u53ef\u80fd\u8bf1\u5bfc\u660e\u663e\u7684\u8870\u8001\u4f5c\u7528\uff0c\u5305\u62ec\u663e\u7740\u589e\u52a0\u8870\u8001\u4eba\u7fa4\u7684\u7aef\u7c92\u957f\u5ea6\u548c\u8870\u8001\u7ec6\u80de\u7684\u6e05\u9664\u7387\u3002<\/p>\n

\u4ecb\u7ecd<\/strong><\/span><\/pre>\n
\u8870\u8001\u7684\u7279\u5f81\u662f\u751f\u7406\u5b8c\u6574\u6027\u7684\u9010\u6e10\u4e27\u5931\uff0c\u5bfc\u81f4\u529f\u80fd\u53d7\u635f\u4ee5\u53ca\u5bf9\u75be\u75c5\u548c\u6b7b\u4ea1\u7684\u654f\u611f\u6027\u3002\u8fd9\u79cd\u751f\u7269\u6076\u5316\u88ab\u8ba4\u4e3a\u662f\u5c24\u5176\u662f\u764c\u75c7\uff0c\u5fc3\u8840\u7ba1\u75be\u75c5\uff0c\u7cd6\u5c3f\u75c5\u548c\u963f\u5c14\u8328\u6d77\u9ed8\u6c0f\u75c5\u7684\u4e3b\u8981\u5371\u9669\u56e0\u7d20\u3002\u5728\u7ec6\u80de\u6c34\u5e73\u4e0a\uff0c\u8870\u8001\u8fc7\u7a0b\u6709\u4e24\u4e2a\u5173\u952e\u6807\u5fd7\uff1a\u7aef\u7c92\u957f\u5ea6\u7684\u7f29\u77ed\u548c\u7ec6\u80de\u8870\u8001[\u00a01<\/a>\u00a0]\u3002<\/p>\n
\u7aef\u7c92\u662f\u4f4d\u4e8e\u67d3\u8272\u4f53\u672b\u7aef\u7684\u4e32\u8054\u6838\u82f7\u9178\u91cd\u590d\u5e8f\u5217\uff0c\u53ef\u7ef4\u6301\u57fa\u56e0\u7ec4\u7a33\u5b9a\u6027\u3002\u7aef\u7c92\u5728\u590d\u5236\uff08\u6709\u4e1d\u5206\u88c2\uff09\u8fc7\u7a0b\u4e2d\u4f1a\u7f29\u77ed\uff0c\u8fd9\u662f\u7531\u4e8e\u56fa\u6709\u7684\u65e0\u6cd5\u5b8c\u5168\u590d\u5236\u843d\u540e\u7684DNA\u94fe\u672b\u7aef\u7684\u539f\u56e0[\u00a02<\/a>\u00a0]\u3002\u7aef\u7c92\u957f\u5ea6\uff08TL\uff09\uff0c4\u81f315\u4e2a\u78b1\u57fa\u4e4b\u95f4\u6d4b\u91cf\uff0c\u9010\u6e10\u7f29\u77ed\u6bcf\u5e74\u7531\u301c20-40\u4e2a\u78b1\u57fa\uff0c\u5e76\u4e0e\u4e0d\u540c\u7684\u75be\u75c5\uff0c\u4f4e\u7269\u7406\u6027\u80fd\u548c\u5927\u8111[\u7684\u76ae\u8d28\u53d8\u8584\u76f8\u5173\u80543<\/a>\u00a0–\u00a05<\/a>\u00a0]\u3002\u5f53TL\u8fbe\u5230\u4e34\u754c\u957f\u5ea6\u65f6\uff0c\u7ec6\u80de\u5c06\u65e0\u6cd5\u590d\u5236\u5e76\u53d1\u5c55\u4e3a\u8870\u8001\u6216\u7a0b\u5e8f\u6027\u7ec6\u80de\u6b7b\u4ea1[\u00a06<\/a>\u00a0]\u3002Goglin\u7b49\u3002\u8bc1\u660e\uff0c\u62e5\u6709\u66f4\u77edTL\u7684\u6210\u5e74\u4eba\u6b7b\u4ea1\u7387\u589e\u52a0[\u00a07<\/a>]\u3002\u7f29\u77edTLS\u53ef\u76f4\u63a5\u9057\u4f20\u6027\u72b6\uff0c\u4f46\u4e00\u4e9b\u73af\u5883\u56e0\u7d20\u4e5f\u4e0e\u7f29\u77edTL\u5305\u62ec\u5e94\u6fc0\u76f8\u5173\uff0c\u7f3a\u4e4f\u8eab\u4f53\u8010\u529b\u6d3b\u52a8\uff0c\u8fc7\u91cf\u8eab\u4f53\u8d28\u91cf\u6307\u6570\uff0c\u5438\u70df\uff0c\u6162\u6027\u708e\u75c7\uff0c\u7ef4\u751f\u7d20\u7f3a\u4e4f\u548c\u6c27\u5316\u5e94\u6fc0\u76842<\/a>\uff0c8<\/a>\uff0c9<\/a>\u00a0]\u3002<\/p>\n
\u7ec6\u80de\u8870\u8001\u662f\u7ec6\u80de\u5468\u671f\u7684\u505c\u6ede\uff0c\u8fd9\u53ef\u80fd\u662f\u7531\u7aef\u7c92\u7f29\u77ed[\u00a010<\/a>\u00a0]\u4ee5\u53ca\u5176\u4ed6\u4e0eTL\u76f8\u5173\u7684\u8870\u8001\u76f8\u5173\u523a\u6fc0\uff08\u4f8b\u5982\u975e\u7aef\u7c92DNA\u635f\u4f24\uff09\u5f15\u8d77\u7684[\u00a01<\/a>\u00a0]\u3002\u8870\u8001\u7684\u4e3b\u8981\u76ee\u7684\u662f\u901a\u8fc7\u89e6\u53d1\u53d7\u635f\u7ec6\u80de\u901a\u8fc7\u514d\u75ab\u7cfb\u7edf\u7684\u6e05\u9664\u6765\u9632\u6b62\u53d7\u635f\u7ec6\u80de\u7684\u7e41\u6b96\u3002\u8870\u8001\u7ec6\u80de\u968f\u7740\u8870\u8001\u7684\u79ef\u7d2f\u53cd\u6620\u51fa\u8fd9\u4e9b\u7ec6\u80de\u7684\u751f\u6210\u589e\u52a0\u548c\/\u6216\u5b83\u4eec\u7684\u6e05\u9664\u7387\u964d\u4f4e\uff0c\u8fd9\u53cd\u8fc7\u6765\u52a0\u5267\u4e86\u635f\u5bb3\u5e76\u52a0\u5267\u4e86\u8870\u8001[\u00a01<\/a>\u00a0]\u3002<\/p>\n
\u8d8a\u6765\u8d8a\u591a\u7684\u7814\u7a76\u8eab\u4f53\u5df2\u7ecf\u53d1\u73b0\u4e86\u51e0\u4e2a\u836f\u7406\u5b66\u8bd5\u5242\uff0c\u53ef\u4ee5\u964d\u4f4e\u7aef\u7c92\u7f29\u77ed\u7387[\u00a011<\/a>\uff0c12<\/a>\u00a0]\u3002\u51e0\u4e2a\u751f\u6d3b\u65b9\u5f0f\u5e72\u9884\u5305\u62ec\u8010\u529b\u8bad\u7ec3\uff0c\u996e\u98df\u548c\u8865\u5145\u5242\u9776\u5411\u7ec6\u80de\u4ee3\u8c22\u548c\u6c27\u5316\u5e94\u6fc0\u5df2\u7ecf\u62a5\u9053\u76f8\u5bf9\u5c0f\u7684\u6548\u679c\uff082-5\uff05\uff09\u4e0aTL\u00a03\uff0c<\/sup>\u00a0[\u00a02<\/a>\uff0c8<\/a>\uff0c9<\/a>\u00a0]\u3002<\/p>\n
\u9ad8\u538b\u6c27\u7597\u6cd5\uff08HBOT\uff09\u5728\u9ad8\u4e8e\u4e00\u4e2a\u7edd\u5bf9\u5927\u6c14\u538b\uff08ATA\uff09\u7684\u73af\u5883\u538b\u529b\u4e0b\u5229\u7528100\uff05\u7684\u6c27\u6c14\u6765\u589e\u52a0\u6eb6\u89e3\u5728\u4eba\u4f53\u7ec4\u7ec7\u4e2d\u7684\u6c27\u6c14\u91cf\u3002\u91cd\u590d\u95f4\u6b47\u9ad8\u6c27\u66b4\u9732\uff0c\u4f7f\u7528\u67d0\u4e9bHBOT\u534f\u8bae\uff0c\u53ef\u8bf1\u5bfc\u901a\u5e38\u5728\u9ad8\u6c27\u73af\u5883\u7f3a\u6c27\u65f6\u53d1\u751f\uff0c\u6240\u8c13\u7684\u9ad8\u6c27\u7f3a\u6c27\u6096\u8bba[\u751f\u7406\u6548\u5e9413<\/a>\u00a0–\u00a016<\/a>\u00a0]\u3002\u6b64\u5916\uff0c\u6700\u8fd1\u6709\u7814\u7a76\u8868\u660e\uff0cHBOT\u53ef\u4ee5\u901a\u8fc7\u6d89\u53ca\u8111\u8840\u6d41\u533a\u57df\u53d8\u5316\u7684\u673a\u5236\u6765\u8bf1\u5bfc\u5065\u5eb7\u8870\u8001\u6210\u5e74\u4eba\u7684\u8ba4\u77e5\u589e\u5f3a[\u00a017]\u3002<\/a>]\u3002\u5728\u7ec6\u80de\u6c34\u5e73\u4e0a\uff0c\u5df2\u8bc1\u660eHBOT\u53ef\u4ee5\u8bf1\u5bfc\u7f3a\u6c27\u8bf1\u5bfc\u56e0\u5b50\uff08HIF\uff09\uff0c\u8840\u7ba1\u5185\u76ae\u751f\u957f\u56e0\u5b50\uff08VEGF\uff09\u548csirtuin\uff08SIRT\uff09\u7684\u8868\u8fbe\uff0c\u5e72\u7ec6\u80de\u589e\u6b96\uff0c\u7ebf\u7c92\u4f53\u751f\u7269\u53d1\u751f\uff0c\u8840\u7ba1\u751f\u6210\u548c\u795e\u7ecf\u53d1\u751f[\u00a018<\/a>\u00a0]\u3002\u4f46\u662f\uff0c\u8fc4\u4eca\u4e3a\u6b62\uff0c\u5c1a\u65e0\u7814\u7a76\u68c0\u67e5HBOT\u5bf9TL\u548c\u8870\u8001\u7ec6\u80de\u79ef\u7d2f\u7684\u5f71\u54cd\u3002<\/p>\n
\u5f53\u524d\u7814\u7a76\u7684\u76ee\u7684\u662f\u8bc4\u4f30HBOT\u662f\u5426\u4f1a\u5f71\u54cd\u6210\u5e74\u4eba\u7684TL\u548c\u8870\u8001\u6837T\u7ec6\u80de\u3002<\/p>\n
\u7ed3\u679c<\/span><\/strong><\/pre>\n
35\u4e2a\u4eba\u88ab\u5206\u914d\u5230HBOT\u3002\u4e94\u540d\u60a3\u8005\u672a\u5b8c\u6210\u57fa\u7ebf\u8bc4\u4f30\uff0c\u88ab\u6392\u9664\u5728\u5916\u3002\u6240\u6709\u5b8c\u6210\u57fa\u7ebf\u8bc4\u4f30\u768430\u540d\u60a3\u8005\u5747\u5b8c\u6210\u4e86\u5e72\u9884\u63aa\u65bd\u3002\u7531\u4e8e\u8840\u6db2\u6837\u672c\u8d28\u91cf\u4f4e\uff08\u7ec6\u80de\u6570\u91cf\u5c11\u6216\u6280\u672f\u4eba\u5458\u9519\u8bef\uff09\uff0c\u7aef\u7c92\u5206\u6790\u4e2d\u6392\u9664\u4e864\u4f8b\u60a3\u8005\uff0c\u8870\u8001\u7ec6\u80de\u5206\u6790\u4e2d\u6392\u9664\u4e8610\u4f8b\u60a3\u8005\uff08\u56fe1<\/a>\uff09\u3002\u88681<\/a>\u63d0\u4f9b\u4e86\u60a3\u8005\u88ab\u6392\u9664\u540e\u7684\u57fa\u7ebf\u7279\u5f81\u548c\u961f\u5217\u6bd4\u8f83\u3002\u4e09\u7ec4\u4e4b\u95f4\u65e0\u663e\u7740\u5dee\u5f02\uff08\u88681<\/a>\uff09\u3002<\/p>\n
\n
\"\u60a3\u8005\u6d41\u7a0b\u56fe\u3002\"<\/a><\/p>\n
\u56fe1.<\/b>\u00a0\u60a3\u8005\u6d41\u7a0b\u56fe\u3002<\/b><\/p>\n<\/div>\n
\n
\u88681.\u57fa\u7ebf\u7279\u5f81\u3002<\/h4>\n<\/div>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\nHBOT<\/b><\/td>\n\u7aef\u7c92\u5206\u6790<\/b><\/td>\n\u8870\u8001\u5206\u6790<\/b><\/td>\nP\u503c<\/b><\/td>\n<\/tr>\n
\u00f1<\/b><\/td>\n<\/td>\n30<\/td>\n25\uff0883.3\uff05\uff09<\/td>\n20\uff0866.6\uff05\uff09<\/td>\n<\/td>\n<\/tr>\n
\u5e74\u9f84\uff08\u5c81\uff09<\/b><\/td>\n<\/td>\n68.41\u00b113.2<\/td>\n67.56\u00b114.35<\/td>\n66.70\u00b116.00<\/td>\n0.917<\/td>\n<\/tr>\n
\u4f53\u91cd\u6307\u6570<\/b><\/td>\n<\/td>\n26.77\u00b13.20<\/td>\n26.89\u00b13.34<\/td>\n27.14\u00b13.81<\/td>\n0.946<\/td>\n<\/tr>\n
\u96c4\u6027<\/b><\/td>\n<\/td>\n16\uff0853.3\uff05\uff09<\/td>\n13\uff0852.0\uff05\uff09<\/td>\n10\uff0850.0\uff05\uff09<\/td>\n0.987<\/td>\n<\/tr>\n
\u96cc\u6027<\/b><\/td>\n<\/td>\n14\uff0847.7\uff05\uff09<\/td>\n12\uff0848.0\uff05\uff09<\/td>\n10\uff0850.0\uff05\uff09<\/td>\n0.987<\/td>\n<\/tr>\n
\u5168\u8840\u7ec6\u80de\u8ba1\u6570<\/b><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n\u8840\u7ea2\u86cb\u767d<\/td>\n6.33\u00b11.25<\/td>\n6.57\u00b11.15<\/td>\n6.58\u00b11.29<\/td>\n0.707<\/td>\n<\/tr>\n
<\/td>\n\u767d\u8840\u7ec6\u80de<\/td>\n14.02\u00b11.40<\/td>\n13.92\u00b11.35<\/td>\n13.97\u00b11.49<\/td>\n0.969<\/td>\n<\/tr>\n
<\/td>\n\uff05PBMC<\/td>\n39.96\u00b16.75<\/td>\n39.25\u00b16.64<\/td>\n38.59\u00b16.63<\/td>\n0.774<\/td>\n<\/tr>\n
<\/td>\n\u8840\u5c0f\u677f<\/td>\n239.87\u00b11.39<\/td>\n244.08\u00b143.0<\/td>\n254.05\u00b141.4<\/td>\n0.559<\/td>\n<\/tr>\n
\u6162\u6027\u75c5<\/b><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n\u5fc3\u623f\u98a4\u52a8<\/td>\n4\uff0813.3\uff05\uff09<\/td>\n4\uff0816.0\uff05\uff09<\/td>\n2\uff0810.0\uff05\uff09<\/td>\n0.841<\/td>\n<\/tr>\n
<\/td>\n\u7532\u72b6\u817a\u529f\u80fd\u51cf\u9000<\/td>\n4\uff0813.3\uff05\uff09<\/td>\n4\uff0816.0\uff05\uff09<\/td>\n3\uff0815.8\uff05\uff09<\/td>\n0.956<\/td>\n<\/tr>\n
<\/td>\n\u963b\u585e\u6027\u7761\u7720\u547c\u5438\u6682\u505c<\/td>\n4\uff0813.3\uff05\uff09<\/td>\n4\uff0816.0\uff05\uff09<\/td>\n3\uff0815.0\uff05\uff09<\/td>\n0.961<\/td>\n<\/tr>\n
<\/td>\n\u54ee\u5598<\/td>\n1\uff083.3\uff05\uff09<\/td>\n1\uff084.0\uff05\uff09<\/td>\n0<\/td>\n0.680<\/td>\n<\/tr>\n
<\/td>\n\u524d\u5217\u817a\u589e\u751f\u75c7<\/td>\n7\uff0823.3\uff05\uff09<\/td>\n5\uff0820.0\uff05\uff09<\/td>\n6\uff0830.0\uff05\uff09<\/td>\n0.733<\/td>\n<\/tr>\n
<\/td>\n\u683c\u5c14\u5fb7<\/td>\n3\uff0810\uff05\uff09<\/td>\n2\uff088.0\uff05\uff09<\/td>\n2\uff0810.0\uff05\uff09<\/td>\n0.961<\/td>\n<\/tr>\n
<\/td>\n\u9aa8\u8d28\u758f\u677e\u75c7<\/td>\n5\uff0816.7\uff05\uff09<\/td>\n5\uff0820.0\uff05\uff09<\/td>\n4\uff0820.0\uff05\uff09<\/td>\n0.936<\/td>\n<\/tr>\n
<\/td>\n\u98ce\u6e7f\u6027\u5173\u8282\u708e<\/td>\n1\uff083.3\uff05\uff09<\/td>\n0<\/td>\n1\uff085.0\uff05\uff09<\/td>\n0.561<\/td>\n<\/tr>\n
<\/td>\n\u9aa8\u5173\u8282\u708e<\/td>\n7\uff0823.3\uff05\uff09<\/td>\n4\uff0816.0\uff05\uff09<\/td>\n5\uff0825.0\uff05\uff09<\/td>\n0.755<\/td>\n<\/tr>\n
<\/td>\n\u7cd6\u5c3f\u75c5<\/td>\n3\uff0810\uff05\uff09<\/td>\n3\uff0812.0\uff05\uff09<\/td>\n2\uff0810.0\uff05\uff09<\/td>\n0.966<\/td>\n<\/tr>\n
<\/td>\n\u9ad8\u8840\u538b<\/td>\n7\uff0823.3\uff05\uff09<\/td>\n5\uff0820.0\uff05\uff09<\/td>\n5\uff0825.0\uff05\uff09<\/td>\n0.918<\/td>\n<\/tr>\n
<\/td>\n\u8840\u8102\u5f02\u5e38<\/td>\n16\uff0853.3\uff05\uff09<\/td>\n14\uff0856.0\uff05\uff09<\/td>\n12\uff0860.0\uff05\uff09<\/td>\n0.897<\/td>\n<\/tr>\n
<\/td>\n\u7f3a\u8840\u6027\u5fc3\u810f\u75c5<\/td>\n2\uff086.7\uff05\uff09<\/td>\n1\uff084.0\uff05\uff09<\/td>\n2\uff0810.0\uff05\uff09<\/td>\n0.725<\/td>\n<\/tr>\n
<\/td>\n\u5438\u70df\u53f2<\/td>\n10\uff0833.3\uff05\uff09<\/td>\n8\uff0832.0\uff05\uff09<\/td>\n7\uff0835.0\uff05\uff09<\/td>\n0.978<\/td>\n<\/tr>\n
\u6162\u6027\u836f\u7269<\/b><\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n\u53cd\u805a\u96c6<\/td>\n8\uff0826.7\uff05\uff09<\/td>\n6\uff0824.0\uff05\uff09<\/td>\n5\uff0825.0\uff05\uff09<\/td>\n0.974<\/td>\n<\/tr>\n
<\/td>\nACE\u6291\u5236\u5242\/ ARB\u963b\u6ede\u5242<\/td>\n6\uff0820\uff05\uff09<\/td>\n6\uff0824.0\uff05\uff09<\/td>\n6\uff0830.0\uff05\uff09<\/td>\n0.720<\/td>\n<\/tr>\n
<\/td>\nBeta\u963b\u6ede\u5242<\/td>\n5\uff0816.7\uff05\uff09<\/td>\n5\uff0820.0\uff05\uff09<\/td>\n3\uff0815.0\uff05\uff09<\/td>\n0.901<\/td>\n<\/tr>\n
<\/td>\n\u9499\u963b\u6ede\u5242<\/td>\n3\uff0810\uff05\uff09<\/td>\n3\uff0812.0\uff05\uff09<\/td>\n2\uff0810.0\uff05\uff09<\/td>\n0.966<\/td>\n<\/tr>\n
<\/td>\n\u963f\u5c14\u6cd5\u963b\u6ede\u5242<\/td>\n7\uff0823.3\uff05\uff09<\/td>\n5\uff0820.0\uff05\uff09<\/td>\n6\uff0830.0\uff05\uff09<\/td>\n0.733<\/td>\n<\/tr>\n
<\/td>\n\u5229\u5c3f\u5242<\/td>\n2\uff086.7\uff05\uff09<\/td>\n1\uff084.0\uff05\uff09<\/td>\n1\uff085.0\uff05\uff09<\/td>\n0.906<\/td>\n<\/tr>\n
<\/td>\n\u4ed6\u6c40\u7c7b<\/td>\n10\uff0833.3\uff05\uff09<\/td>\n9\uff0836.0\uff05\uff09<\/td>\n7\uff0835.0\uff05\uff09<\/td>\n0.978<\/td>\n<\/tr>\n
<\/td>\n\u53e3\u670d\u964d\u7cd6<\/td>\n1\uff083.3\uff05\uff09<\/td>\n1\uff084.0\uff05\uff09<\/td>\n1\uff085.0\uff05\uff09<\/td>\n0.958<\/td>\n<\/tr>\n
<\/td>\n\u53cc\u81a6\u9178\u76d0<\/td>\n1\uff083.3\uff05\uff09<\/td>\n1\uff084.0\uff05\uff09<\/td>\n1\uff085.0\uff05\uff09<\/td>\n0.958<\/td>\n<\/tr>\n
<\/td>\n\u8d28\u5b50\u6cf5\u6291\u5236\u5242<\/td>\n3\uff0810\uff05\uff09<\/td>\n3\uff0812.0\uff05\uff09<\/td>\n3\uff0815.0\uff05\uff09<\/td>\n0.726<\/td>\n<\/tr>\n
<\/td>\n\u6fc0\u7d20<\/td>\n3\uff0810\uff05\uff09<\/td>\n3\uff0812.0\uff05\uff09<\/td>\n2\uff0810.0\uff05\uff09<\/td>\n0.966<\/td>\n<\/tr>\n
<\/td>\n\u82ef\u4e8c\u6c2e\u5353\u7c7b<\/td>\n3\uff0810\uff05\uff09<\/td>\n2\uff088.0\uff05\uff09<\/td>\n1\uff085.0\uff05\uff09<\/td>\n0.816<\/td>\n<\/tr>\n
<\/td>\n\u7cbe\u795e\u75c5\u7814\u7a76\u6240<\/td>\n5\uff0816.7\uff05\uff09<\/td>\n5\uff0820.0\uff05\uff09<\/td>\n3\uff0815.0\uff05\uff09<\/td>\n0.990<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
\n
\u7aef\u7c92\u957f\u5ea6<\/h3>\n
\u4e0e\u57fa\u7ebf\u76f8\u6bd4\uff0c\u7b2c30\u5c4a<\/sup>\u4f1a\u8bae\u548cHBOT\u540eT\u8f85\u52a9\u7aef\u7c92\u957f\u5ea6\u663e\u7740\u589e\u52a021.70\u00b140.05\uff08p = 0.042\uff09\uff0c23.69\uff05\u00b139.54\uff08p = 0.012\uff09\u548c29.30\u00b138.51\uff08p = 0.005\uff09 \uff09\uff08\u56fe2<\/a>\uff09\u3002\u4f46\u662f\uff0c\u91cd\u590d\u6d4b\u91cf\u5206\u6790\u663e\u793a\u51fa\u4e0d\u663e\u7740\u7684\u8d8b\u52bf\uff08F = 4.663\uff0cp = 0.06\uff0c\u88682<\/a>\u548c\u56fe2<\/a>\uff09\u3002<\/p>\n
\n
\"\u7aef\u7c92\u957f\u5ea6\u968fHBOT\u53d8\u5316\u3002<\/a><\/p>\n
\u56fe2.<\/b>\u00a0\u7aef\u7c92\u957f\u5ea6\u968fHBOT\u53d8\u5316\u3002<\/b>\u5e73\u5747\u503c+ SEM * p \uff1c0.05\uff0c** p \uff1c0.01\uff0c*** p \uff1c0.001\u3002<\/p>\n<\/div>\n
\n
\u88682. HBOT\u540e\u7aef\u7c92\u957f\u5ea6\u548c\u8870\u8001\u7ec6\u80de\u7684\u53d8\u5316\u3002<\/h4>\n
\"\"<\/p>\n
\u4e0e\u57fa\u7ebf\u76f8\u6bd4\uff0cB\u7ec6\u80de\u7684\u7aef\u7c92\u957f\u5ea6\u572830\u663e\u8457\u589e\u52a0\u6b21<\/sup>\u4f1a\u8bae\u4e0a\uff0c60\u4e2a<\/sup>\u4f1a\u8bae\u548c25.68\uff05-HBOT\u4ea4\u00b140.42\uff08p\u503c= 0.007\uff09\uff0c29.39\uff05\u00b123.39\uff08p\u503c= 0.0001\uff09\u548c37.63\uff05\u00b152.73\uff08 p = 0.007\uff09\uff08\u56fe2<\/a>\uff09\u3002\u91cd\u590d\u6d4b\u91cf\u5206\u6790\u663e\u793a\u4e86\u663e\u7740\u7684\u7ec4\u5185\u6548\u5e94\uff08F = 0.390\uff0cp = 0.017\uff0c\u88682<\/a>\u548c\u56fe2<\/a>\uff09\u3002<\/p>\n<\/div>\n
\u4e0e\u57fa\u7ebf\u76f8\u6bd4\uff0c\u7b2c30\u5c4a<\/sup>\u4f1a\u8bae\uff08p = 0.045\uff09\u548c\u7b2c60\u5c4a<\/sup>\u4f1a\u8bae\u7684\u81ea\u7136\u6740\u4f24\u7ec6\u80de\u7aef\u7c92\u957f\u5ea6\u663e\u7740\u589e\u52a0\u4e8620.56\uff05\u00b133.35\uff08p = 0.013\uff09\u3002HBOT\u540e\uff0cHBOT\u540e\u7aef\u7c92\u957f\u5ea6\u589e\u52a0\u4e8622.16\uff05\u00b144.81\uff08p = 0.06\uff0c\u88682<\/a>\u548c\u56fe2<\/a>\uff09\u3002\u91cd\u590d\u6d4b\u91cf\u5206\u6790\u8868\u660e\uff0c\u5728\u7b2c30\u4e2a<\/sup>\u7597\u7a0b\u540e\u6ca1\u6709\u5176\u4ed6\u663e\u7740\u5f71\u54cd\uff08F = 0.812\uff0cp = 0.391\uff09\u3002<\/p>\n
\u4e0e\u57fa\u7ebf\u76f8\u6bd4\uff0c\u7ec6\u80de\u6bd2\u6027T\u7ec6\u80de\u5728\u7b2c30\u5c4a<\/sup>\u4f1a\u8bae\u4e0a\u6ca1\u6709\u663e\u7740\u589e\u52a0\uff0c\u589e\u5e45\u4e3a18.29\uff05\u00b145.62\uff08p = 0.11\uff09\uff0c\u968f\u540e\u5728\u7b2c60\u5c4a<\/sup>\u4f1a\u8bae\u4e0a\u663e\u7740\u589e\u52a0\u4e8624.13\uff05\u00b140.88\u00a0\uff08p = 0.0019\uff09 HBOT\u540e\u4e3a19.59\uff05\u00b133.98\uff08p = 0.023\uff09\u3002\u91cd\u590d\u6d4b\u91cf\u5206\u6790\u8868\u660e\uff0c\u5728\u7b2c30\u4e2a<\/sup>\u7597\u7a0b\u540e\uff0c\u6ca1\u6709\u5176\u4ed6\u660e\u663e\u7684\u5f71\u54cd\uff08F = 1.159\uff0cp = 0.310\uff0c\u88682<\/a>\u548c\u56fe2<\/a>\uff09\u3002<\/p>\n<\/div>\n
\n
\u8870\u8001\u7ec6\u80de<\/h3>\n
\u6709\u5728\u8870\u8001T-\u8f85\u52a9\u7ec6\u80de\u7684\u6570\u91cf\u572830\u975e\u663e\u8457\u4e0b\u964d\u4e2a<\/sup>\u4f1a\u8bdd\uff0c\u5e76\u4e1460\u4e2a<\/sup>\u5206\u522b\u7531-19.66\uff05\u00b180.03\uff08p\u503c= 0.09\uff09\u548c-11.67\uff05\u00b194.30\uff08p\u503c= 0.20\uff09\u4f1a\u8bdd\u3002\u4f46\u662f\uff0cHBOT\u540e\u8870\u8001\u7684T\u8f85\u52a9\u8005\u6570\u91cf\u663e\u7740\u4e0b\u964d-37.30\uff05\u00b133.04\uff08P <0.0001\uff0c\u56fe3<\/a>\uff09\u3002\u91cd\u590d\u6d4b\u91cf\u5206\u6790\u663e\u793a\uff0c\u5373\u4f7f\u5728\u7b2c30\u6b21\u6cbb\u7597<\/sup>\u540e\u4ecd\u5177\u6709\u663e\u7740\u7684\u8fde\u7eed\u4f5c\u7528\uff0c\u5e76\u5177\u6709\u7ec4\u5185\u4f5c\u7528\uff08F = 8.547\uff0cp = 0.01\uff0c\u88682<\/a>\u548c\u56fe3<\/a>\uff09\u3002<\/p>\n
\n
\"HBOT\u5bfc\u81f4\u8870\u8001\u7ec6\u80de\u53d1\u751f\u53d8\u5316\u3002<\/a><\/p>\n
\u56fe3.<\/b>\u00a0HBOT\u5f15\u8d77\u7684\u8870\u8001\u7ec6\u80de\u53d8\u5316\u3002<\/b>\u5e73\u5747\u503c+ SEM * p \uff1c0.05\uff0c** p \uff1c0.01\uff0c*** p \uff1c0.001\u3002<\/p>\n<\/div>\n
\u5728\u7b2c30\u5c4a<\/sup>HBOT\u4f1a\u8bae\u4e0a\uff0cT\u7ec6\u80de\u6bd2\u6027\u8870\u8001\u7ec6\u80de\u767e\u5206\u6bd4\u663e\u7740\u4e0b\u964d-12.21\uff05\u00b18.74\uff08P <0.0001\uff09\uff0c\u5728\u7b2c<\/sup>60\u5c4a<\/sup>HBOT\u4f1a\u8bae\u4e0a\uff080.002\uff09\u4e0b\u964d-9.81\uff05\u00b19.50\u548c-10.96\uff05\u00b112.59\uff08p = 0.0004\uff09 HBOT\u540e\uff09\uff08\u88682<\/a>\u548c\u56fe3<\/a>\uff09\u3002\u91cd\u590d\u6d4b\u91cf\u5206\u6790\u663e\u793a\uff0c\u5373\u4f7f\u5728\u7b2c30\u6b21\u6cbb\u7597<\/sup>\u540e\u4ecd\u5177\u6709\u663e\u7740\u7684\u8fde\u7eed\u4f5c\u7528\uff0c\u5177\u6709\u7ec4\u5185\u6548\u5e94\uff08F = 6.916\uff0cp = 0.018\uff0c\u88682<\/a>\uff09\u3002<\/p>\n<\/div>\n
\n
HIF-1\u03b1<\/h3>\n
\u5728\u7b2c60\u5c4a<\/sup>\u4f1a\u8bae\u4e0a\uff0cHIF-1alpha\u6c34\u5e73\u4ece10.54\u00b13.39\u589e\u52a0\u523019.71\u00b13.39\u00a0\uff08p = 0.006\uff09\uff0c\u5176\u4e2dHBOT\u540e2\u5468\u7684\u6c34\u5e73\u4e3a16.81\u00b17.65\uff0c\u4e0e\u57fa\u7ebf\u65e0\u663e\u7740\u5dee\u5f02\uff08p = 0.16\uff09\u3002<\/p>\n<\/div>\n
\u8ba8\u8bba<\/span><\/strong><\/pre>\n
\u5728\u8fd9\u9879\u7814\u7a76\u4e2d\uff0c\u9996\u6b21\u5728\u4eba\u7c7b\u4e2d\u53d1\u73b0\uff0c\u5728\u8001\u9f84\u5316\u4eba\u7fa4\u4e2d\uff0c\u6bcf\u5929\u91cd\u590d\u8fdb\u884cHBOT\u7597\u7a0b\u53ef\u4f7fPBMC\u7aef\u7c92\u957f\u5ea6\u589e\u52a020\uff05\u4ee5\u4e0a\uff0c\u5176\u4e2dB\u7ec6\u80de\u7684\u53d8\u5316\u6700\u4e3a\u663e\u7740\u3002\u6b64\u5916\uff0cHBOT\u4f7f\u8870\u8001\u7ec6\u80de\u7684\u6570\u91cf\u51cf\u5c11\u4e8610-37\uff05\uff0c\u5176\u4e2dT\u8f85\u52a9\u8870\u8001\u7ec6\u80de\u53d7\u5230\u7684\u5f71\u54cd\u6700\u5927\u3002<\/p>\n
\u5df2\u7ecf\u89c2\u5bdf\u5230\u7aef\u7c92\u957f\u5ea6\u548c\u751f\u6d3b\u65b9\u5f0f\u6539\u53d8\u4e4b\u95f4\u7684\u5927\u91cf\u5173\u8054\u3002\u8fd9\u5bfc\u81f4\u4e86\u51e0\u9879\u5e72\u9884\u6027\u7814\u7a76\uff0c\u5305\u62ec\u996e\u98df\uff0c\u8865\u54c1\uff08\u4f8b\u5982omega-3\u548c\u6838\u6843\uff09\uff0c\u4f53\u80b2\u953b\u70bc\uff0c\u538b\u529b\u7ba1\u7406\u548c\u793e\u4f1a\u652f\u6301\u3002\u4e00\u9879\u5bf9\u8ba4\u77e5\u5065\u5eb7\u7684\u8001\u5e74\u4eba\u8fdb\u884c\u7684\u4e3a\u671f\u4e24\u5e74\u7684\u8bd5\u9a8c\uff0c\u4f7f\u7528\u5bcc\u542b\u6838\u6843\u7684\u996e\u98df\uff0c\u4e0e\u5bf9\u7167\u996e\u98df\u76f8\u6bd4\uff0c\u4fdd\u7559\u7aef\u7c92\u957f\u5ea6\u7684\u8d8b\u52bf\u4e0d\u660e\u663e[\u00a019<\/a>\u00a0]\u3002\u5728\u53e6\u4e00\u9879\u8bc4\u4f30\u5bf9\u8001\u5e74\u4eba\u8fdb\u884c\u5341\u4e8c\u5468\u4f4e\u9891\u7206\u70b8\u6027\u62b5\u6297\u8bad\u7ec3\u7684\u6548\u679c\u7684\u7814\u7a76\u4e2d\uff0c\u5e72\u9884\u7ec4\u7684\u7aef\u7c92\u957f\u5ea6\u5f97\u5230\u4e86\u66f4\u597d\u7684\u4fdd\u7559\uff0c\u800c\u6ca1\u6709\u663e\u7740\u589e\u52a0[\u00a020]\u3002<\/a>]\u3002\u6700\u8fd1\u7684\u4e00\u9879\u7814\u7a76\u53d1\u73b0\uff0c\u6709\u6c27\u8010\u529b\u8bad\u7ec3\u6216\u516d\u4e2a\u6708\u7684\u9ad8\u5f3a\u5ea6\u95f4\u6b47\u8bad\u7ec3\u53ef\u4f7f\u7aef\u7c92\u957f\u5ea6\u589e\u52a05\uff05[\u00a021<\/a>\u00a0]\u3002\u989d\u5916\u7684\u51cf\u80a5\uff0c\u745c\u4f3d\u548c\u538b\u529b\u7ba1\u7406\u6280\u672f\u672a\u80fd\u663e\u793a\u7aef\u7c92\u957f\u5ea6\u7684\u663e\u7740\u53d8\u5316[\u00a022<\/a>\u00a0\u2013\u00a025<\/a>\u00a0]\u3002\u7136\u800c\uff0c\u5927\u591a\u6570\u8fd9\u4e9b\u7814\u7a76\u5df2\u663e\u793a\u6297\u6c27\u5316\u6d3b\u6027\u548c\u7aef\u7c92\u9176\u6d3b\u6027[\u4e4b\u95f4\u663e\u8457\u76f8\u5173\u602722<\/a>\u00a0–\u00a025<\/a>\u00a0]\u3002<\/p>\n
\u5c3d\u7ba1\u8bb8\u591a\u9057\u4f20\u548c\u73af\u5883\u56e0\u7d20\u4e0e\u7aef\u7c92\u7f29\u77ed\u6709\u5173\uff0c\u4f46\u6700\u5e38\u89c1\u7684\u63d0\u793a\u673a\u5236\u662f\u6c27\u5316\u5e94\u6fc0\u3002\u6c27\u5316\u5e94\u6fc0\u53ef\u80fd\u662f\u7531\u4e8e\u6d3b\u6027\u6c27\uff08ROS\uff09\u4e0e\u7ec6\u80de\u6e05\u9664\u5242\u7684\u4ea7\u751f\u4e0d\u5e73\u8861\u800c\u5f15\u8d77\u7684\u3002\u7aef\u7c92\u5bf9\u6c27\u5316\u6027DNA\u635f\u4f24\u9ad8\u5ea6\u654f\u611f\uff0c\u540e\u8005\u53ef\u5bfc\u81f4\u7aef\u7c92\u7f29\u77ed\u548c\u529f\u80fd\u969c\u788d[\u00a026<\/a>\u00a0]\u3002\u6c27\u548c\/\u6216\u6c27\u5316\u5e94\u6fc0\u4e0e\u7aef\u7c92\u957f\u5ea6\u4e4b\u95f4\u7684\u5173\u7cfb\u5728\u8fc7\u53bb\u7684\u51e0\u5341\u5e74\u4e2d\u4e00\u76f4\u5b58\u5728\u4e89\u8bae\u3002\u4eba\u7c7b\u7ec6\u80de\u57f9\u517b\u7814\u7a76\u4e00\u81f4\u8868\u660e\uff0c\u8f7b\u5ea6\u7684\u6c27\u5316\u5e94\u6fc0\u4f1a\u52a0\u901f\u7aef\u7c92\u7f29\u77ed\uff0c\u800c\u6297\u6c27\u5316\u5242\u548c\u81ea\u7531\u57fa\u6e05\u9664\u5242\u4f1a\u964d\u4f4e\u7f29\u77ed\u901f\u7387\u5e76\u5ef6\u957f\u7ec6\u80de\u589e\u6b96\u5bff\u547d[\u00a027<\/a>]\u3002\u5bf9\u75c5\u7406\u72b6\u51b5\uff08\u4f8b\u5982\u7cd6\u5c3f\u75c5\uff0c\u708e\u6027\u75be\u75c5\uff0c\u5e15\u91d1\u68ee\u6c0f\u75c5\uff09\u7684\u4e00\u4e9b\u4e34\u5e8a\u7814\u7a76\u8868\u660e\uff0c\u6c27\u5316\u5e94\u6fc0\u6807\u8bb0\uff0c\u6d3b\u6027\u6c27\u6e05\u9664\u5242\u6c34\u5e73\u548c\u7aef\u7c92\u957f\u5ea6\u4e4b\u95f4\u5b58\u5728\u76f8\u5173\u6027[\u00a028<\/a>\u00a0]\u3002\u7136\u800c\uff0c\u5065\u5eb7\u7684\u4e2a\u4f53\u6ca1\u6709\u663e\u793a\u51fa\u76f8\u4f3c\u7684\u7ed3\u679c[\u00a029<\/a>\u00a0]\u3002<\/p>\n
\u4ee5\u524d\u5df2\u7ecf\u6709\u4eba\u63d0\u51fa\u5c06\u7ec6\u80de\u57f9\u517b\u7269\u66b4\u9732\u4e8e\u9ad8\u538b\u73af\u5883\u4e2d\u4f1a\u8bf1\u5bfc\u660e\u663e\u7684\u6c27\u5316\u5e94\u6fc0\u548c\u8fc7\u65e9\u7684\u7ec6\u80de\u8870\u8001[\u00a030<\/a>\u00a0]\u3002\u4f46\u662f\uff0c\u8fd9\u662f\u57fa\u4e8e\u9ad8\u538b\u57f9\u517b\u7bb1\u4e2d\u751f\u957f\u7684\u5206\u79bb\u7ec6\u80de\uff0c\u800c\u4e0d\u662f\u50cf\u672c\u7814\u7a76\u4e2d\u90a3\u6837\u57fa\u4e8e\u4eba\u7c7b\u7684\u590d\u6742\u751f\u7269\u7cfb\u7edf\u3002\u4e0e\u76ee\u524d\u7684\u7814\u7a76\u76f8\u4f3c\uff0c\u5148\u524d\u5bf9\u66b4\u9732\u4e8e\u5f3a\u9ad8\u538b\u6c27\u7684\u6f5c\u6c34\u8005\u8fdb\u884c\u7684\u4e3a\u671f\u4e00\u5e74\u7684\u524d\u77bb\u6027\u89c2\u5bdf\u7814\u7a76\u663e\u793a\uff0c\u767d\u7ec6\u80de\u4e2d\u7aef\u7c92\u7684\u4f38\u957f\u663e\u7740[\u00a031<\/a>\u00a0]\u3002\u5982\u5728\u5f53\u524d\u7684\u7814\u7a76\u4e2d\u6240\u4f7f\u7528\u7684\uff0c\u9ad8\u538b\u6c27\u534f\u8bae\u5229\u7528\u7531\u91cd\u590d\u95f4\u6b47\u9ad8\u6c27\u66b4\u9732\u81f4\u7684\u5f71\u54cd\uff0c\u5373\u6240\u8c13\u7684\u9ad8\u6c27\u7f3a\u6c27\u6096\u8bba[\u00a013<\/a>\uff0c18<\/a>]\u3002\u8fd9\u4e9b\u95f4\u6b47\u6027\u7684\u9ad8\u6c27\u66b4\u9732\u5f15\u8d77\u9002\u5e94\u6027\u53cd\u5e94\uff0c\u5305\u62ec\u589e\u52a0\u6297\u6c27\u5316\u5242\u57fa\u56e0\u7684\u4e0a\u8c03[\u00a032<\/a>\u00a0]\u548c\u4ea7\u751f\u6297\u6c27\u5316\u5242\/\u6e05\u9664\u5242\uff0c\u4ee5\u9002\u5e94\u589e\u52a0\u7684ROS\u751f\u6210\uff0c\u4ece\u800c\u5bfc\u81f4ROS \/\u6e05\u9664\u5242\u7684\u6bd4\u4f8b\u9010\u6e10\u53d8\u5f97\u4e0e\u6b63\u5e38\u6c27\u6c14\u73af\u5883\u4e0b\u7684\u6bd4\u4f8b\u76f8\u4f3c\u3002\u4f46\u662f\uff0c\u7531\u4e8e\u6e05\u9664\u5242\u6d88\u9664\u534a\u8870\u671f\uff08T\u00a01\/2<\/sub>\uff09\u660e\u663e\u5927\u4e8eROS\u7684T\u00a01\/2<\/sub>\uff0c\u56e0\u6b64\u5728\u91cd\u590d\u5e38\u9ad8\u6c27\u66b4\u9732\u540e\uff0c\u6062\u590d\u6b63\u5e38\u6c27\u6c34\u5e73\u540e\uff0c\u6e05\u9664\u5242\u7684\u6c34\u5e73\u663e\u7740\u63d0\u9ad8\uff0c\u6297\u6c27\u5316\u6d3b\u6027\u63d0\u9ad8[\u00a013]<\/a>\uff0c18<\/a>]\u3002\u56e0\u6b64\uff0c\u7c7b\u4f3c\u4e8e\u4f53\u80b2\u953b\u70bc\u548c\u70ed\u91cf\u9650\u5236\uff0c\u6bcf\u5929\u91cd\u590d\u8fdb\u884cHBOT\u534f\u8bae\u53ef\u80fd\u4f1a\u5f15\u8d77\u5174\u594b\u73b0\u8c61\u3002\u5355\u4e2a\u66dd\u5149\u589e\u52a0ROS\u4ea7\u751f\u5267\u70c8\uff0c\u5f15\u53d1\u6297\u6c27\u5316\u53cd\u5e94\uff0c\u5e76\u7528\u91cd\u590d\u66b4\u9732\uff0c\u54cd\u5e94\u53d8\u5f97\u4fdd\u62a4[\u00a013<\/a>\uff0c18<\/a>\u00a0]\u3002<\/p>\n
\u53e6\u5916\uff0c\u95f4\u6b47\u6027\u7684\u9ad8\u6c27\u66b4\u9732\u5f15\u8d77\u8bb8\u591a\u5728\u7f3a\u6c27\u671f\u95f4\u53d1\u751f\u7684\u751f\u7406\u53cd\u5e94[\u00a013<\/a>\u00a0]\u3002HBOT\u8bf1\u5bfc\u79f0\u4e3a\u4f4e\u6c27\u8bf1\u5bfc\u56e0\u5b50\uff08HIF\uff09\u7684\u8f6c\u5f55\u56e0\u5b50\u7684\u91ca\u653e\uff0c\u5e76\u589e\u52a0\u5176\u7a33\u5b9a\u6027\u548c\u6d3b\u6027[\u00a014<\/a>\u00a0]\u3002\u53cd\u8fc7\u6765\uff0cHIF\u8bf1\u5bfc\u7ec6\u80de\u7ea7\u8054\u53cd\u5e94\uff0c\u5305\u62ec\u8840\u7ba1\u5185\u76ae\u751f\u957f\u56e0\u5b50\u548c\u8840\u7ba1\u751f\u6210\u8bf1\u5bfc\uff0c\u7ebf\u7c92\u4f53\u751f\u7269\u53d1\u751f\uff0c\u5e72\u7ec6\u80de\u52a8\u5458\u548cSIRT1\u6d3b\u6027\u589e\u52a0[\u00a018<\/a>\u00a0]\u3002\u6211\u4eec\u7684\u7814\u7a76\u8bc1\u5b9e\uff0c\u91cd\u590d\u7684HBOT\u66b4\u9732\u4f1a\u8bf1\u5bfcHIF\u8868\u8fbe\u589e\u52a0\uff0c\u800c\u5728\u975e\u5355\u58f0\u73af\u5883\u4e0b\uff0cHOT\u7684\u6c34\u5e73\u4f1a\u968f\u7740\u6b63\u5e38\u6c34\u5e73\u7684\u964d\u4f4e\u800c\u9010\u6e10\u964d\u4f4e\u3002<\/p>\n
\u76ee\u524d\uff0c\u5728\u52a8\u7269\u6a21\u578b\u4e2d\u5df2\u5f00\u53d1\u51fa\u8bb8\u591a\u901a\u8fc7\u9057\u4f20\u6216\u836f\u7406\u5b66\u65b9\u6cd5\uff08\u8870\u8001\u836f\uff09\u53bb\u9664\u8870\u8001\u7ec6\u80de\u7684\u5e72\u9884\u63aa\u65bd\uff0c\u5e76\u6b63\u5728\u7b49\u5f85\u4eba\u7c7b\u7684\u5b89\u5168\u6027\u548c\u6709\u6548\u6027\u8bc4\u4f30[\u00a033<\/a>\u00a0]\u3002\u5f53\u524d\u7684\u7814\u7a76\u63d0\u51fa\u4e86\u4e00\u79cd\u975e\u836f\u7406\u5b66\u65b9\u6cd5\uff0c\u53ef\u7528\u4e8e\u51cf\u5c11\u8870\u8001\u7ec6\u80de\u6570\u91cf\u7684\u4e34\u5e8a\u5b89\u5168\u6027\u6982\u51b5\u3002\u6211\u4eec\u7684\u534f\u8bae\u5305\u62ec\u57282 ATA\u8fdb\u884c60\u6b21100\uff05\u7684\u6c27\u6c14\u5904\u7406\uff0c\u5305\u62ec\u6bcf\u6b21\u4f7f\u7528\u4e09\u4e2a\u7a7a\u6c14\u4e2d\u65ad\uff0c\u4ee5\u5229\u7528\u9ad8\u6c27\u4f4e\u6c27\u6096\u8bba\u5e76\u4f7f\u6c27\u6c14\u4e2d\u6bd2\u7684\u98ce\u9669\u6700\u5c0f\u5316\u3002\u6709\u8da3\u7684\u662f\uff0cTL\u548c\u8870\u8001\u7ec6\u80de\u51cf\u5c11\u5cf0\u503c\u572830\u4e2a<\/sup>\u4f1a\u8bae\u3002\u4f46\u662f\uff0c\u4e0e\u65bd\u52a0\u7684\u538b\u529b\uff0c\u65f6\u95f4\u548cHBOT\u66b4\u9732\u6b21\u6570\u6709\u5173\u7684\u5242\u91cf\u53cd\u5e94\u66f2\u7ebf\u53ca\u5176\u4e0eHIF\u8868\u8fbe\u53ca\u5176\u76f8\u5173\u7684\u518d\u751f\u6548\u5e94\u7684\u5173\u7cfb\u4ecd\u4e0d\u5b8c\u5168\u6e05\u695a\uff0c\u9700\u8981\u8fdb\u4e00\u6b65\u7814\u7a76\u4ee5\u627e\u5230\u6700\u4f73\u7684HBOT\u65b9\u6848\u3002<\/p>\n
\u9ad8\u538b\u6c27\u7597\u6cd5\u662f\u4e00\u79cd\u9488\u5bf9\u975e\u6108\u5408\u4f24\u53e3\uff0c\u653e\u5c04\u7ebf\u635f\u4f24\u4ee5\u53ca\u4e0d\u540c\u7684\u7f3a\u6c27\u6216\u7f3a\u8840\u4e8b\u4ef6\uff08\u4f8b\u5982\u4e00\u6c27\u5316\u78b3\u4e2d\u6bd2\uff0c\u611f\u67d3\u7b49\uff09\u7684\u884c\u4e4b\u6709\u6548\u7684\u6cbb\u7597\u65b9\u5f0f\u3002\u8fd1\u5e74\u6765\uff0c\u4ece\u4e34\u5e8a\u524d\u4e00\u4e2a\u8d8a\u6765\u8d8a\u591a\u7684\u8bc1\u636e\u4ee5\u53ca\u4e34\u5e8a\u8bd5\u9a8c\u8868\u660e\u9ad8\u538b\u6c27\u7528\u4e8e\u6cbb\u7597\u795e\u7ecf\u9002\u5e94\u75c7\uff0c\u5305\u62ec\u7279\u53d1\u6027\u7a81\u53d1\u6027\u611f\u89c9\u795e\u7ecf\u6027\u542c\u529b\u635f\u5931[\u529f\u654834<\/a>\u00a0]\uff0c\u5352\u4e2d\u540e\u548c\u540e\u521b\u4f24\u6027\u8111\u635f\u4f24[\u00a035<\/a>\u00a0–\u00a041<\/a>\u00a0]\uff0c\u4e2d\u67a2\u81f4\u654f\u7efc\u5408\u5f81\u5982\u7ea4\u7ef4\u808c\u75db\u7efc\u5408\u5f81[\u00a042<\/a>\uff0c43<\/a>\u00a0]\u548c\u5e74\u9f84\u76f8\u5173\u7684\u8ba4\u77e5\u8870\u9000[\u00a017<\/a>\u00a0]\u548c\u52a8\u7269\u963f\u5c14\u8328\u6d77\u9ed8\u6c0f\u75c5\u7684\u6a21\u578b[\u00a044<\/a>]\u3002\u672c\u7814\u7a76\u9996\u6b21\u65e8\u5728\u8bc4\u4f30\u65e0\u4efb\u4f55\u529f\u80fd\u53d7\u9650\u75be\u75c5\u7684\u8870\u8001\u4eba\u7c7b\u5bf9\u7ec6\u80de\u6c34\u5e73\u7684\u751f\u7406\u5f71\u54cd\u3002<\/p>\n
\n
\u7814\u7a76\u5c40\u9650\u6027<\/h3>\n
\u5f53\u524d\u7684\u7814\u7a76\u6709\u51e0\u4e2a\u5c40\u9650\u6027\u548c\u9700\u8981\u8003\u8651\u7684\u4f18\u70b9\u3002\u9996\u5148\uff0c\u5fc5\u987b\u8003\u8651\u6709\u9650\u7684\u6837\u672c\u91cf\u3002\u7b2c\u4e8c\uff0c\u7f3a\u4e4f\u5bf9\u7167\u7ec4\u3002\u4f46\u662f\uff0c\u8fd9\u9879\u7814\u7a76\u8868\u660e\uff0c\u5728TL\u548c\u8870\u8001\u7ec6\u80de\u6e05\u9664\u65b9\u9762\uff0c\u4ee4\u4eba\u5370\u8c61\u6df1\u523b\u7684\u7ed3\u679c\u5728\u5176\u4ed6\u5e72\u9884\u63aa\u65bd\u4e2d\u5747\u672a\u89c2\u5bdf\u5230\u3002\u6b64\u5916\uff0c\u6211\u4eec\u961f\u5217\u7684\u57fa\u7ebf\u7aef\u7c92\u957f\u5ea6\u503c\u4e0e\u8001\u9f84\u5316\u4eba\u7fa4\u7684\u9884\u671f\u503c[\u00a045<\/a>\u00a0\u2013\u00a047<\/a>]\u3002\u7b2c\u4e09\uff0c\u6548\u679c\u7684\u6301\u7eed\u65f6\u95f4\u6709\u5f85\u957f\u671f\u968f\u8bbf\u786e\u5b9a\u3002\u7b2c\u56db\uff0c\u7531\u4e8e\u9009\u62e9\u4e86\u7528\u4e8e\u8840\u6db2\u4fdd\u5b58\u548c\u8bc4\u4f30\u7684\u65b9\u6cd5\uff0c\u56e0\u6b64\u672a\u8bc4\u4f30\u7aef\u7c92\u9176\u6d3b\u6027\u3002\u4f46\u662f\uff0c\u5e94\u8be5\u5f3a\u8c03\u4e00\u4e9b\u4f18\u52bf\u3002\u5728\u8fd9\u9879\u7814\u7a76\u4e2d\uff0cCD28\u7528\u4f5c\u8870\u8001\u7ec6\u80de\u7684\u751f\u7269\u6807\u5fd7\u7269\uff0c\u800cCD57\u4e0d\u80fd\u7528\u4f5cT\u7ec6\u80de\u8870\u8001\u7684\u786e\u8ba4\u6027\u6807\u5fd7\u7269\u3002\u5728\u7279\u5b9a\u7684\u767d\u7ec6\u80de\u7fa4\u4f53\u4e0a\u8bc4\u4f30\u751f\u7269\u6807\u5fd7\u7269\uff0c\u800c\u4e0d\u662f\u5c06\u6574\u4e2aPBMC\u7528\u4f5c\u4e00\u7ec4\u3002\u5bf9\u5b64\u7acb\u7684HBOT\u6548\u679c\u8fdb\u884c\u4e86\u6d4b\u91cf\uff0c\u5e76\u5bf9\u53c2\u4e0e\u8005\u8fdb\u884c\u4e86\u76d1\u63a7\uff0c\u4ee5\u786e\u4fdd\u4ed6\u4eec\u6ca1\u6709\u6539\u53d8\u751f\u6d3b\u65b9\u5f0f\uff08\u5982\u8425\u517b\u548c\u8fd0\u52a8\uff09\uff0c\u7528\u836f\u6216\u4efb\u4f55\u5176\u4ed6\u53ef\u80fd\u9020\u6210\u6df7\u6dc6\u7684\u5e72\u9884\u63aa\u65bd\u3002<\/p>\n
\u603b\u800c\u8a00\u4e4b\uff0c\u8be5\u7814\u7a76\u8868\u660e\uff0cHBOT\u53ef\u4ee5\u8bf1\u5bfc\u660e\u663e\u7684\u8870\u8001\u4f5c\u7528\uff0c\u5305\u62ec\u7aef\u7c92\u957f\u5ea6\u7684\u663e\u7740\u589e\u52a0\u548c\u8870\u8001\u7fa4\u4f53\u4e2d\u8870\u8001\u7ec6\u80de\u7684\u6e05\u9664\u3002<\/p>\n<\/div>\n
\u6750\u6599\u548c\u65b9\u6cd5<\/span><\/strong><\/pre>\n
\n
\u79d1\u76ee<\/h3>\n
\u5165\u9009\u4e86\u4e09\u5341\u4e94\u540d\u672a\u53d1\u751f\u75c5\u7406\u6027\u8ba4\u77e5\u51cf\u9000\u7684\u6210\u5e74\u4eba\uff0c\u5e74\u9f84\u572864\u5c81\u53ca\u4ee5\u4e0a\uff0c\u4ed6\u4eec\u4ee5\u826f\u597d\u7684\u529f\u80fd\u548c\u8ba4\u77e5\u72b6\u6001\u72ec\u7acb\u751f\u6d3b\u3002\u8be5\u7814\u7a76\u57282016\u5e74\u81f32020\u5e74\u4e4b\u95f4\u5728\u4ee5\u8272\u5217\u7684Shamir\uff08Assaf-Harofeh\uff09\u533b\u7597\u4e2d\u5fc3\u8fdb\u884c\u3002\u88ab\u7eb3\u5165\u7684\u60a3\u8005\u5728\u88ab\u7eb3\u5165\u4e4b\u524d\u7684\u6700\u540e\u4e00\u5e74\u6ca1\u6709\u5fc3\u810f\u6216\u8111\u8840\u7ba1\u5c40\u90e8\u7f3a\u8840\u7684\u75c5\u53f2\u3002\u6392\u9664\u6807\u51c6\u5305\u62ec\uff1a\u5728\u8fc7\u53bb\u4e09\u4e2a\u6708\u4e2d\u56e0\u4efb\u4f55\u539f\u56e0\u5148\u524d\u63a5\u53d7\u8fc7HBOT\u6cbb\u7597\uff0c\u5728\u8fc7\u53bb\u4e00\u5e74\u4e2d\u6709\u4efb\u4f55\u6076\u6027\u75c5\u53f2\uff0c\u4efb\u4f55\u75c5\u7406\u6027\u8ba4\u77e5\u529f\u80fd\u4e0b\u964d\uff0c\u4e25\u91cd\u7684\u6162\u6027\u80be\u8870\u7aed\uff08GFR <30\uff09\uff0c\u4e0d\u53d7\u63a7\u5236\u7684\u7cd6\u5c3f\u75c5\uff08HbA1C> 8\uff0c\u7981\u98df\uff09\u8840\u7cd6> 200\uff09\uff0c\u514d\u75ab\u6291\u5236\u5242\uff0cMRI\u7981\u5fcc\u75c7\uff08\u5305\u62ecBMI> 35\uff09\uff0c\u79ef\u6781\u5438\u70df\u6216\u80ba\u90e8\u75be\u75c5\u3002<\/p>\n<\/div>\n
\n
\u5b66\u4e60\u89c4\u5212<\/h3>\n
\u8be5\u7814\u7a76\u65b9\u6848\u5df2\u83b7\u5f97\u4ee5\u8272\u5217\u6c99\u7c73\u5c14\u533b\u5b66\u4e2d\u5fc3\u7684\u673a\u6784\u5ba1\u67e5\u59d4\u5458\u4f1a\u7684\u6279\u51c6\u3002\u8fd9\u9879\u7814\u7a76\u662f\u4e00\u9879\u524d\u77bb\u6027\u4e34\u5e8a\u8bd5\u9a8c\u3002\u7b7e\u7f72\u77e5\u60c5\u540c\u610f\u4e66\u5e76\u8fdb\u884c\u57fa\u7ebf\u8bc4\u4f30\u540e\uff0c\u5c06\u53d7\u8bd5\u8005\u5206\u914d\u5230HBOT\u3002\u5728\u57fa\u7ebf\uff0c\u6cbb\u7597\u65b9\u6848\u7684\u4e00\u534a\uff08\u7b2c<\/sup>30\u6b21<\/sup>\u6cbb\u7597\uff09\uff0c\u6700\u540e\u4e00\u6b21HBOT\u6cbb\u7597\u7684\u5f53\u5929\u4ee5\u53caHBOT\u6cbb\u7597\u540e1-2\u5468\u8bc4\u4f30\u6d4b\u91cf\u70b9\u3002<\/p>\n
\u8be5\u7814\u7a76\u961f\u5217\u4ec5\u5305\u62ec\u63a5\u53d7HBOT\u6cbb\u7597\u7684\u60a3\u8005\uff0c\u8fd9\u662f\u5728\u4ee5\u8272\u5217Shamir\u533b\u5b66\u4e2d\u5fc3\u7814\u7a76\u7684\u8f83\u5927\u7684\u6b63\u5e38\u8870\u8001\u4eba\u7fa4\u7684\u4e00\u90e8\u5206\uff08NCT02790541 [\u00a017<\/a>\u00a0]\uff09\u3002<\/p>\n<\/div>\n
\n
\u5e72\u9884\u63aa\u65bd<\/h3>\n
HBOT\u534f\u8bae\u5728Multiplace Starmed-2700\u5b9e\u9a8c\u5ba4\uff08\u5fb7\u56fdHAUX\uff09\u4e2d\u8fdb\u884c\u7ba1\u7406\u3002\u8be5\u534f\u8bae\u5305\u62ec60\u4e2a\u6bcf\u65e5\u8bfe\u7a0b\uff0c\u4e09\u4e2a\u6708\u5185\u6bcf\u5468\u4e94\u4e2a\u8bfe\u7a0b\u3002\u6bcf\u8282\u8bfe\u5305\u62ec\u57282ATA\u5904\u901a\u8fc7\u53e3\u7f69\u547c\u5438100\uff05\u7684\u6c27\u6c1490\u5206\u949f\uff0c\u6bcf20\u5206\u949f\u4f11\u606f5\u5206\u949f\u3002\u538b\u7f29\/\u51cf\u538b\u901f\u7387\u4e3a1\u7c73\/\u5206\u949f\u3002\u5728\u8bd5\u9a8c\u671f\u95f4\uff0c\u4e0d\u5141\u8bb8\u6539\u53d8\u751f\u6d3b\u65b9\u5f0f\u548c\u996e\u98df\u4e60\u60ef\uff0c\u4e5f\u4e0d\u5141\u8bb8\u8c03\u6574\u836f\u7269\u6cbb\u7597\u3002<\/p>\n<\/div>\n
\n
\u8840\u6db2\u6837\u672c<\/h3>\n
\u4f7f\u7528\u6807\u51c6\u6280\u672f\uff0c\u5728\u57fa\u7ebf\uff0cHBOT\u65b9\u6848\u7684\u4e00\u534a\uff08\u7b2c<\/sup>30\u4e2a<\/sup>\u7597\u7a0b\uff09\uff0c\u6700\u540e\u4e00\u6b21HBOT\u7597\u7a0b\u7684\u4e00\u5929\uff08\u7b2c<\/sup>60\u4e2a<\/sup>\u7597\u7a0b\uff09\u548c\u672f\u540e1-2\u5468\u5c06\u5168\u8840\u6837\u54c1\u6536\u96c6\u5230EDTA\u8bd5\u7ba1\u4e2d\u3002\u4e0a\u6b21HBOT\u4f1a\u8bdd\u3002<\/p>\n<\/div>\n
\n
\u5916\u5468\u8840\u5355\u6838\u7ec6\u80de\uff08PBMC\uff09\u5206\u79bb<\/h3>\n
\u4f7f\u7528\u78f7\u9178\u76d0\u7f13\u51b2\u76d0\u6c34\uff08PBS\uff09\u7a00\u91ca\u5168\u8840\u3002\u4f7f\u7528\u88c5\u6ee1Lymphoprep\u7684Leucosep\u7ba1\u8fdb\u884c\u5bc6\u5ea6\u68af\u5ea6\u5206\u79bb\u3002\u7136\u540e\u5c06\u8bd5\u7ba1\u572825\u00b0C\u4e0b\u4ee51000x\u00a0g<\/i>\u79bb\u5fc310\u5206\u949f\u3002\u79bb\u5fc3\u540e\uff0c\u7acb\u5373\u901a\u8fc7\u79fb\u6db2\u7ba1\u6536\u96c6\u7ec6\u80de\u5c42\uff08\u8840\u6c89\u68d5\u9ec4\u5c42\uff09\uff0c\u5e76\u8f6c\u79fb\u81f350 mL\u9525\u5f62\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u7528\u8db3\u591f\u76841X PBS\u91cd\u60ac\u81f350 mL\u7684\u4f53\u79ef\uff0c\u5e76\u5728300x\u00a0g<\/i>\u4e0b\u4e8e25\u00b0C\u79bb\u5fc310\u5206\u949f\u3002\u9664\u53bb\u4e0a\u6e05\u6db2\u540e\uff0c\u6807\u8bb0\u6bcf\u4e2a\u6837\u54c1\u3002<\/p>\n<\/div>\n
\n
\u7aef\u7c92\u957f\u5ea6<\/h3>\n
\u6839\u636eDako PNA \/ FITC\u8bd5\u5242\u76d2\u89c4\u7a0b\uff08\u4ee3\u7801K5327\uff09\u6807\u8bb0\u7aef\u7c92\u3002\u5728\u7531PBMC\uff08\u6837\u54c1\u7ec6\u80de\uff09\u548cTCL 1301\u7ec6\u80de\u7cfb\uff08\u5bf9\u7167\u7ec6\u80de\uff09\u7684\u6df7\u5408\u7269\u7ec4\u6210\u7684\u5355\u7ec6\u80de\u60ac\u6db2\u4e2d\uff0c\u5728\u6ca1\u6709\u63a2\u9488\u7684\u6742\u4ea4\u6eb6\u6db2\u5b58\u5728\u4e0b\uff0c\u5728\u5fae\u91cf\u79bb\u5fc3\u7ba1\u4e2d\u4e8e82\u00b0C\u5c06DNA\u53d8\u602710\u5206\u949f\u3002\u5728\u542b\u6709\u8367\u5149\u7d20\u7f00\u5408\u7684PNA\u7aef\u7c92\u63a2\u9488\u7684\u6742\u4ea4\u6eb6\u6db2\u4e2d\u8fdb\u884c\u7535\u6cf3\u3002\u6742\u4ea4\u5728\u9ed1\u6697\u4e2d\u5728\u5ba4\u6e29\uff08RT\uff09\u4e0b\u8fdb\u884c\u8fc7\u591c\u3002\u6742\u4ea4\u540e\uff0c\u572840\u00b0C\u7528\u6d17\u6da4\u6eb6\u6db2\u8fdb\u884c\u4e24\u6b2110\u5206\u949f\u7684\u6742\u4ea4\u540e\u6742\u4ea4\u3002\u7136\u540e\u5728\u9002\u5f53\u7684\u7f13\u51b2\u6db2\u4e2d\u7528CD4 +\uff0cCD8 +\uff0cCD3 +\uff0cCD19 +\u548cCD56 +\u7f00\u5408\u7684\u6297\u4f53\u6807\u8bb0\u6837\u54c1\uff0c\u4ee5\u8fdb\u884c\u8fdb\u4e00\u6b65\u7684\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790[\u00a048]<\/a>\uff0c49<\/a>]\u3002\u6bcf\u4e2a\u6837\u54c1\u4e00\u5f0f\u4e24\u4efd\u8fd0\u884c\u3002\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790\u540e\uff0c\u8ba1\u7b97CD3 + \/ CD4 +\uff08T-\u8f85\u52a9\uff09\uff0cCD3 + \/ CD8 +\uff08T-\u7ec6\u80de\u6bd2\u6027\uff09\uff0cCD3 + \/ CD56 +\uff08\u5929\u7136\u6740\u4f24\u529b\uff09\u548cCD19 +\uff08B\u7ec6\u80de\uff09\u7684\u76f8\u5bf9\u7aef\u7c92\u957f\u5ea6\uff08RTL\uff09\u3002\u5c06RTL\u503c\u8ba1\u7b97\u4e3a\u6bcf\u4e2a\u6837\u54c1\u7684\u7aef\u7c92\u4fe1\u53f7\u4e0e\u5bf9\u7167\u7ec6\u80de\uff08TCL 1301\u7ec6\u80de\u7cfb\uff09\u4e4b\u95f4\u7684\u6bd4\u7387\uff0c\u5e76\u6821\u6b63\u4e86G0 \/ 1\u7ec6\u80de\u7684DNA\u6307\u6570\u3002\u4f7f\u7528\u7898\u5316\u4e19\u5576\u67d3\u8272\u5206\u522b\u5206\u6790\u6837\u54c1\u7ec6\u80de\u548c\u5bf9\u7167\u7ec6\u80de\u7684DNA\u500d\u6027\uff0c\u4ee5\u6807\u51c6\u5316\u6bcf\u4e2a\u7ec6\u80de\u7684\u7aef\u7c92\u672b\u7aef\u6570\uff0c\u4ece\u800c\u6807\u51c6\u5316\u6bcf\u4e2a\u67d3\u8272\u4f53\u7684\u7aef\u7c92\u957f\u5ea6\u3002\u6709\u5173FACS\u5206\u6790\u793a\u4f8b\uff0c\u8bf7\u53c2\u89c1\u56fe4<\/a>\u3002<\/p>\n
\n
\"T\u8f85\u52a9\u7ec6\u80de\u4e9a\u7fa4\u7684\u6d41\u9c7c\u6570\u636e\u5206\u6790\u5b9e\u4f8b\u3002<\/a><\/p>\n
\u56fe4.<\/b>\u00a0T\u8f85\u52a9\u4e9a\u7fa4\u7684\u6d41\u9c7c\u6570\u636e\u5206\u6790\u793a\u4f8b\u3002<\/b>\u5728\u6570\u636e\u91c7\u96c6\u4e4b\u524d\uff0c\u5c06\u6bcf\u4e2a\u8840\u6db2\u6837\u672c\u7528PNA\u63a2\u9488\uff08\u00a0b<\/b>\uff09\u67d3\u8272\u6216\u4e0d\u4f7f\u7528\uff08\u00a0a<\/b>\uff09\u67d3\u8272\uff0c\u7136\u540e\u8fdb\u884c\u6297\u4f53\u67d3\u8272\uff08CD3\uff0cCD4\uff0cCD8\uff0cCD16\uff0cCD19\uff09\u3002<\/p>\n<\/div>\n<\/div>\n
\n
\u514d\u75ab\u5206\u578b<\/h3>\n
\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u786e\u5b9aCD3 + CD4 + CD28-\u65e0\u6548\u7684T\u7ec6\u80de\uff08\u8870\u8001\u7684T\u8f85\u52a9\u7ec6\u80de\uff09\u548cCD3 + CD8 + CD28-\u65e0\u6548\u7684T\u7ec6\u80de\uff08\u8870\u8001\u7684T\u7ec6\u80de\u6bd2\u6027\uff09\u7684\u767e\u5206\u6bd4\u3002PBMC\u7528VioBlue\u5171\u8f6d\u6297CD3\uff0cViogreen\u5171\u8f6d\u6297CD8\uff0cPE-VIO 770A\u5171\u8f6d\u6297CD4\u548cAPC-VIO 770A\u6297CD28\u6297\u4f53\uff08Miltenyi Biotec\uff09\u67d3\u8272\u3002\u7528MACSQuant\u6d41\u5f0f\u7ec6\u80de\u4eea\uff08Miltenyi Biotec\uff09\u5206\u6790\u7ec6\u80de\u3002\u7136\u540e\u8ba1\u7b97CD4 +\u6216CD8 + T\u7ec6\u80de\u7fa4\u4e2dCD28null T\u7ec6\u80de\u7684\u767e\u5206\u6bd4\u3002<\/p>\n<\/div>\n
\n
\u7f3a\u6c27\u8bf1\u5bfc\u56e0\u5b50\uff08HIF-1alpha\uff09<\/h3>\n
\u56fa\u5b9a\u548c\u901a\u900f\u5316\uff08Life Technologies\uff09\u540e\uff0c<\/i>\u7528APC\u5076\u8054\u7684\u6297HIF1a\u6297\u4f53\u6216\u76f8\u5e94\u7684\u540c\u578b\u5bf9\u7167\uff08R\uff06D\u7cfb\u7edf\uff09\u8fdb\u884c\u7ec6\u80de\u5185HIF1a\u67d3\u8272\u3002<\/i>\u7528MACSQuant\u6d41\u5f0f\u7ec6\u80de\u4eea\uff08Miltenyi Biotec\uff09\u5206\u6790\u7ec6\u80de\uff0c\u5e76\u786e\u5b9a\u8868\u8fbeHIF1a\u7684PBMC\u7684\u767e\u5206\u6bd4\u3002<\/p>\n<\/div>\n
\n
\u7edf\u8ba1\u5206\u6790<\/h3>\n
\u9664\u975e\u53e6\u6709\u8bf4\u660e\uff0c\u5426\u5219\u8fde\u7eed\u6570\u636e\u8868\u793a\u4e3a\u5747\u503c\u00b1\u6807\u51c6\u5dee\u3002\u4f7f\u7528Kolmogorov-Smirnov\u68c0\u9a8c\u5bf9\u6240\u6709\u53d8\u91cf\u7684\u6b63\u6001\u5206\u5e03\u8fdb\u884c\u4e86\u68c0\u9a8c\u3002\u8fdb\u884c\u4e86\u5355\u5411\u65b9\u5dee\u5206\u6790\uff0c\u4ee5\u6bd4\u8f83\u57fa\u7ebf\u65f6\u4e09\u7ec4\u4e4b\u95f4\u548c\u4e4b\u5185\u7684\u53d8\u91cf\u3002<\/p>\n
\u5206\u7c7b\u6570\u636e\u4ee5\u6570\u5b57\u548c\u767e\u5206\u6bd4\u8868\u793a\uff0c\u5e76\u901a\u8fc7\u5361\u65b9\u68c0\u9a8c\u8fdb\u884c\u6bd4\u8f83\u3002\u4f7f\u7528Chi-Square \/ Fisher\u7cbe\u786e\u68c0\u9a8c\u8fdb\u884c\u5355\u53d8\u91cf\u5206\u6790\uff0c\u4ee5\u8bc6\u522b\u663e\u7740\u53d8\u91cf\uff08P <0.05\uff09\u3002<\/p>\n
\u4e3a\u4e86\u8bc4\u4f30HBOT\u7684\u4f5c\u7528\uff0c\u4f7f\u7528\u91cd\u590d\u6d4b\u91cfANOVA\u6a21\u578b\u6765\u6d4b\u8bd5\u4e3b\u8981\u7684\u53d7\u8bd5\u8005\u5185\u4f5c\u7528\u3002\u4f7f\u7528\u5e26\u6709Bonferroni\u6821\u6b63\u7684t\u68c0\u9a8c\u5bf9\u65b9\u6cd5\u8fdb\u884c\u4e8b\u540e\u68c0\u9a8c\uff0c\u4ee5\u68c0\u9a8c\u65f6\u95f4\u5dee\u5f02\u3002<\/p>\n<\/div>\n
 <\/p>\n
 <\/p>\n","protected":false},"excerpt":{"rendered":"
\u65b0\u95fb https:\/\/www.medsci.cn\/article\/show_article.do?id=a34 … \u7ee7\u7eed\u9605\u8bfb\u9ad8\u538b\u6c27\u7597\u6cd5\u53ef\u589e\u52a0\u7aef\u7c92\u7684\u957f\u5ea6\u5e76\u51cf\u5c11\u79bb\u4f53\u8840\u7ec6\u80de\u7684\u514d\u75ab\u8870\u8001\uff1a\u4e00\u9879\u524d\u77bb\u6027\u8bd5\u9a8c<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"aside","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-9666","post","type-post","status-publish","format-aside","hentry","category-uncategorized","post_format-post-format-aside"],"_links":{"self":[{"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=\/wp\/v2\/posts\/9666","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9666"}],"version-history":[{"count":4,"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=\/wp\/v2\/posts\/9666\/revisions"}],"predecessor-version":[{"id":11137,"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=\/wp\/v2\/posts\/9666\/revisions\/11137"}],"wp:attachment":[{"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9666"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9666"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/wp.huhualing.net\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9666"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}